Prague Med. Rep. 2014, 115, 24-32

https://doi.org/10.14712/23362936.2014.3

Differential Expression of Inflammation-related Genes after Intense Exercise

Magdalena C. Kimsa1,*, Barbara Strzalka-Mrozik1, Malgorzata W. Kimsa1, Joanna Gola1, Aleksandra Kochanska-Dziurowicz2, Aleksandra Zebrowska3, Urszula Mazurek1

1Department of Molecular Biology, Medical University of Silesia, Sosnowiec, Poland
2Department of Isotope Diagnostics and Radiopharmaceuticals, Medical University of Silesia, Sosnowiec, Poland
3Department of Physiological and Medical Sciences, Academy of Physical Education, Katowice, Poland

The present study focused on the identification of the difference in expression of inflammation-related genes after intense exercise by oligonucleotide microarray methods. This may finally lead to an improved understanding of underlying cellular and molecular mechanism of the immunological alterations in response to exercises. The study group consisted of three healthy road cyclists. Peripheral blood mononuclear cells (PBMCs) were collected pre-exercise, immediately post-exercise and after 15 min of recovery. The analysis of the expression profile of genes related to the inflammation was performed in PBMCs using HG-U133A oligonucleotide microarrays. 4 genes were found to be regulated by more than 2.0-fold (IL1R2, IL2RB, IL8, IL8RB). Venn diagram indicated that only one of differentially expressed genes (TXLNA) remains the same in each comparison. The balance of both pro- and anti-inflammatory cytokines after exercise seems to be important for athletes. Optimal inflammatory and immune response may help optimize exercise regimes, link physical activity with health and diagnose or prevent athletes from overtraining.

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