Prague Med. Rep. 2017, 118, 128-138

https://doi.org/10.14712/23362936.2017.14

5-fluorouracil Toxicity Mechanism Determination in Human Keratinocytes: in vitro Study on HaCaT Cell Line

Jan Hartinger1, Pavel Veselý2, Martin Šíma1, Irena Netíková1, Eva Matoušková3, Luboš Petruželka4

1Department of Clinical Pharmacology and Pharmacy, Institute of Pharmacology, First Faculty of Medicine, Charles University and General University Hospital in Prague, Prague, Czech Republic
2Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic
3Department of Burns Medicine, Third Faculty of Medicine, Charles University and University Hospital Královské Vinohrady, Prague, Czech Republic
4Department of Oncology, First Faculty of Medicine, Charles University and General University Hospital in Prague, Prague, Czech Republic

Received June 29, 2017
Accepted December 5, 2017

References

1. An, Q., Robins, P., Lindahl, T., Barnes, D. E. (2007) 5-fluorouracil incorporated into DNA is excised by the Smug1 DNA glycosylase to reduce drug cytotoxicity. Cancer Res. 67, 940–945. <https://doi.org/10.1158/0008-5472.CAN-06-2960>
2. Atienzar, F. A., Tilmant, K., Gerets, H. H., Toussaint, G., Speeckaert, S., Hanon, E., Depelchin, O., Dhalluin, S. (2011) The use of real-time cell analyzer technology in drug discovery: Defining optimal cell culture conditions and assay reproducibility with different adherent cellular models. J. Biomol. Screen. 16, 575–587. <https://doi.org/10.1177/1087057111402825>
3. Bagrij, T., Kralovanszky, J., Gyergyay, F., Kiss, E., Peters, G. J. (1993) Influence of uridine treatment in mice on the protection of gastrointestinal toxicity caused by 5-fluorouracil. Anticancer Res. 13, 789–793.
4. Barth, J. (2004) Letter to the editor – 5-FU induced palmar-plantar erythrodyesthesia – a hospital pharmacy developed “antidot”. J. Oncol. Pharm. Pract. 10, 57. <https://doi.org/10.1191/1078155204jp120xx>
5. Biganzoli, L., Martin, M., Twelves, C. (2002) Moving forward with capecitabine: a glimpse of the future. Oncologist 7, 29–35 (Suppl. 6).
6. Boukamp, P., Petrussevska, R. T., Breitkreutz, D., Hornung, J., Markham, A., Fusenig, N. E. (1988) Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line. J. Cell Biol. 106, 761–771. <https://doi.org/10.1083/jcb.106.3.761> <PubMed>
7. Codacci-Pisanelli, G., Kralovanszky, J., van der Wilt, C. L., Noordhuis, P., Colofiore, J. R., Martin, D. S., Franchi, F., Peters, G. J. (1997) Modulation of 5-fluorouracil in mice using uridine diphosphoglucose. Clin. Cancer Res. 3, 309–315.
8. Codacci-Pisanelli, G., Noordhuis, P., van der Wilt, C. L., Peters, G. J. (2008) Selective protection by uridine of growth inhibition by 5-fluorouracil (5FU) mediated by 5FU incorporation into RNA, but not the thymidylate synthase mediated growth inhibition by 5FU-leucovorin. Nucleosides Nucleotides Nucleic Acids 27, 733–739. <https://doi.org/10.1080/15257770802145496>
9. Engstrom, P. F., Arnoletti, J. P., Benson, A. B. 3rd, Chen, Y. J., Choti, M. A., Cooper, H. S., Covey, A., Dilawari, R. A., Early, D. S., Enzinger, P. C., Fakih, M. G., Fleshman, J. Jr., Fuchs, C., Grem, J. L., Kiel, K., Knol, J. A., Leong, L. A., Lin, E., Mulcahy, M. F., Rao, S., Ryan, D. P., Saltz, L., Shibata, D., Skibber, J. M., Sofocleous, C., Thomas, J., Venook, A. P., Willett, C. (2009) NCCN Clinical Practice Guidelines in Oncology: colon cancer. J. Natl. Compr. Canc. Netw. 7, 778–831. <https://doi.org/10.6004/jnccn.2009.0056>
10. Hofheinz, R. D., Heinemann, V., von Weikersthal, L. F., Laubender, R. P., Gencer, D., Burkholder, I., Hochhaus, A., Stintzing, S. (2012) Capecitabine-associated hand-foot-skin reaction is an independent clinical predictor of improved survival in patients with colorectal cancer. Br. J. Cancer 107, 1678–1683. <https://doi.org/10.1038/bjc.2012.434> <PubMed>
11. Janusch, M., Fischer, M., Marsch, W. C., Holzhausen, H. J., Kegel, T., Helmbold, P. (2006) The hand-foot syndrome – A frequent secondary manifestation in antineoplastic chemotherapy. Eur. J. Dermatol. 16, 494–499.
12. Leonard, R., Hennessy, B. T., Blum, J. L., O’Shaughnessy, J. (2011) Dose-adjusting capecitabine minimizes adverse effects while maintaining efficacy: a retrospective review of capecitabine for metastatic breast cancer. Clin. Breast Cancer 11, 349–356. <https://doi.org/10.1016/j.clbc.2011.06.005>
13. Lokich, J. J., Ahlgren, J. D., Gullo, J. J., Philips, J. A., Fryer, J. G. (1989) A prospective randomized comparison of continuous infusion fluorouracil with a conventional bolus schedule in metastatic colorectal carcinoma: a Mid-Atlantic Oncology Program Study. J. Clin. Oncol. 7, 425–432. <https://doi.org/10.1200/JCO.1989.7.4.425>
14. Longley, D. B., Harkin, D. P., Johnston, P. G. (2003) 5-fluorouracil: Mechanisms of action and clinical strategies. Nat. Rev. Cancer 3, 330–338. <https://doi.org/10.1038/nrc1074>
15. Martin, D. S., Stolfi, R. L., Sawyer, R. C., Nayak, R., Spiegelman, S., Young, C. W., Woodcock, T. (1980) An overview of thymidine. Cancer 45, 1117–1128. <https://doi.org/10.1002/1097-0142(19800315)45:5+<1117::AID-CNCR2820451316>3.0.CO;2-S>
16. Mosmann, T. (1983) Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods 65, 55–63. <https://doi.org/10.1016/0022-1759(83)90303-4>
17. Netíková, I., Sedláčková, E., Konopásek, B., Petruželka, L. (2009) Therapy of palmar-plantar erythrodysesthesia after continual fluoropyrimidin administration with 10% uridin ointment (ASCO Meeting Abstract). J. Clin. Oncol. 27.
18. Pritchard, D. M., Watson, A. J., Potten, C. S., Jackman, A. L., Hickman, J. A. (1997) Inhibition by uridine but not thymidine of p53-dependent intestinal apoptosis initiated by 5-fluorouracil: evidence for the involvement of RNA perturbation. Proc. Natl. Acad. Sci. U. S. A. 94, 1795–1799. <https://doi.org/10.1073/pnas.94.5.1795> <PubMed>
19. Sawyer, R. C., Stolfi, R. L., Spiegelman, S., Martin, D. S. (1984) Effect of uridine on the metabolism of 5-fluorouracil in the CD8F 1 murine mammary carcinoma system. Pharm. Res. 1, 69–75. <https://doi.org/10.1023/A:1016351330832>
20. Schmoll, H. J., Van Cutsem, E., Stein, A., Valentini, V., Glimelius, B., Haustermans, K., Nordlinger, B., van de Velde, C. J., Balmana, J., Regula, J., Nagtegaal, I. D., Beets-Tan, R. G., Arnold, D., Ciardiello, F., Hoff, P., Kerr, D., Kohne, C. H., Labianca, R., Price, T., Scheithauer, W., Sobrero, A., Tabernero, J., Aderka, D., Barroso, S., Bodoky, G., Douillard, J. Y., El Ghazaly, H., Gallardo, J., Garin, A., Glynne-Jones, R., Jordan, K., Meshcheryakov, A., Papamichail, D., Pfeiffer, P., Souglakos, I., Turhal, S., Cervantes, A. (2012) ESMO Consensus Guidelines for management of patients with colon and rectal cancer. a personalized approach to clinical decision making. Ann. Oncol. 23, 2479–2516. <https://doi.org/10.1093/annonc/mds236>
21. Umeda, M., Heidelberger, C. (1968) Comparative studies of fluorinated pyrimidines with various cell lines. Cancer Res. 28, 2529–2538.
22. van Groeningen, C. J., Peters, G. J., Pinedo, H. M. (1992) Modulation of fluorouracil toxicity with uridine. Semin. Oncol. 19, 148–154.
23. Wolpin, B. M., Mayer, R. J. (2008) Systemic treatment of colorectal cancer. Gastroenterology 134, 1296–1310. <https://doi.org/10.1053/j.gastro.2008.02.098> <PubMed>
24. Wurzer, J. C., Tallarida, R. J., Sirover, M. A. (1994) New mechanism of action of the cancer chemotherapeutic agent 5-fluorouracil in human cells. J. Pharmacol. Exp. Ther. 269, 39–43.
25. Yen-Revollo, J. L., Goldberg, R. M., McLeod, H. L. (2008) Can inhibiting dihydropyrimidine dehydrogenase limit hand-foot syndrome caused by fluoropyrimidines? Clin. Cancer Res. 14, 8–13. <https://doi.org/10.1158/1078-0432.CCR-07-1225>
26. Yun, J. A., Kim, H. C., Son, H. S., Kim, H. R., Yun, H. R., Cho, Y. B., Yun, S. H., Lee, W. Y., Chun, H. K. (2010) Oncologic outcome after cessation or dose reduction of capecitabine in patients with colon cancer. J. Korean Soc. Coloproctol. 26, 287–292. <https://doi.org/10.3393/jksc.2010.26.4.287> <PubMed>
front cover

ISSN 1214-6994 (Print) ISSN 2336-2936 (Online)

Archive