Prague Med. Rep. 2022, 123, 5-19

Effect of Inferior Alveolar Nerve Transection on the Inorganic Component of Molars of Rat Mandible

Ivo Němec1, Václav Smrčka2, Martin Mihaljevič3, Martin Hill4, Jaroslav Pokorný5, Václav Páral6, Ivana Pračková6,7

1Department of Otorhinolaryngology and Maxillofacial Surgery, Third Faculty of Medicine, Charles University and Military University Hospital Prague, Prague, Czech Republic
2Institute for History of Medicine and Foreign Languages, First Faculty of Medicine, Charles University, Prague, Czech Republic
3Institute of Geochemistry, Mineralogy and Mineral Resources, Faculty of Science, Charles University, Prague, Czech Republic
4Institute of Endocrinology, Prague, Czech Republic
5Institute of Physiology, First Faculty of Medicine, Charles University, Prague, Czech Republic
6Department of Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic
7Department of Anatomy, Faculty of Medicine, Masaryk University, Brno, Czech Republic

Received March 9, 2021
Accepted January 31, 2022

The objective of the study was to determine the effects of inferior alveolar nerve transection on inorganic components in mandibular molars of the rat. We used 26 male laboratory rats of the Wistar strain for the study, age 7–9 weeks. The rats were divided in three groups. The control group (intact) included 6 rats. The surgery was performed under general anesthesia. The experimental group included (group with the nerve transected on the left) included 12 rats. The sham group (group with the nerve prepared without transection) included 8 rats. The animals were sacrificed after 4 weeks. Molars from the left and right sides of the mandible were extracted. Element content levels were determined using inductively coupled plasma mass spectrometry. The following elements were determined in all samples: magnesium (Mg), sodium (Na), potassium (K), calcium (Ca), zinc (Zn), and strontium (Sr). The nerve transection caused: a reduction of the contents of Ca and Sr in the mandibular molars; an increase in the contents of Mg and Zn; a difference arrangement of both sides for Na. The surgery approach itself caused a decrease in the contents of Na and K in the experimental and sham groups; the difference in K in M3 between the left and right sides disappeared due to the surgery. Our results have confirmed the hypothesis of inferior alveolar nerve transection having an effect on inorganic components in mandibular molars in the rat.


Part of the equipment used for the study was purchased from the Operational Programme Prague – Competitiveness (Project CZ.2.16/3.1.00/21516). Supported with grant Progres Q35/LF1.


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