Prague Med. Rep. 2026, 127, 151-162
https://doi.org/10.14712/23362936.2026.22
Quantification of PDGF-AB and TGF-β1 Proteins in Platelet-rich Plasma Obtained under Different Conditions
Nikoleta Ivanova
Department of Biology, Faculty of Pharmacy, Medical University of Varna, Varna, Bulgaria
Received September 8, 2025
Accepted September 8, 2026
References
1. , E., Fuente, M. D. L., Merayo-Lloves, J., Muruzabal, F. (2022) Optimization of a plasma rich in growth factors membrane for the treatment of inflammatory ocular diseases. Bioengineering (Basel) 9(10), 508.
<https://doi.org/10.3390/bioengineering9100508>
2. , S. H., Mudalal, M., Al-Aroomi, O. A., Al-Attab, R., Li, W., Yin, L. (2023) The effects of injectable platelet-rich fibrin and advanced-platelet-rich fibrin on gingival fibroblast cell vitality, proliferation, differentiation. Tissue Eng. Regen. Med. 20(7), 1161–1172.
<https://doi.org/10.1007/s13770-023-00586-1>
3. , I., Galea, A. M. (2023) A novel method for the preparation and frozen storage of growth factors and cytokines obtained from platelet-rich plasma. J. Cartil. Jt. Preserv. 3(2), 100089.
4. , J. U., López, C. (2024) Effects of temperature and time on the denaturation of transforming growth factor beta-1 and cytokines from bovine platelet-rich gel supernatants. Gels 10(9), 583.
<https://doi.org/10.3390/gels10090583>
5. , C., Roffi, A., Grigolo, B., Mariani, E., Pratelli, L., Merli, G., Kon, E., Marcacci, M., Filardo, G. (2016) Platelet-rich plasma: The choice of activation method affects the release of bioactive molecules. Biomed Res. Int. 2016, 6591717.
<https://doi.org/10.1155/2016/6591717>
6. Chen, C., Lei, J., Yi, X., Hua, Y., Yang, J. (2023) Investigation of the effect of anticoagulants on platelet recovery, enrichment factor, PDGF, TGF-β1 and optimal dose in the preparation of platelet-rich plasma. Panminerva Med. (Epub ahead of print)
7. , M., Witkowski, J., Moreira, H., Płaczkowska, S., Morasiewicz, P., Reichert, P., Królikowska, A. (2022) Content of blood cell components, inflammatory cytokines and growth factors in autologous platelet-rich plasma obtained by various methods. World J. Orthop. 13(6), 587.
<https://doi.org/10.5312/wjo.v13.i6.587>
8. , M., Feola, A., Ambrosio, P., Pinto, F., Galasso, G., Zarrelli, A., Di Fabio, G., Porcelli, M., Scacco, S., Inchingolo, F., Quagliuolo, L., Ballini, A., Boccellino, M. (2020) Antioxidant effect of beer polyphenols and their bioavailability in dental-derived stem cells (D-dSCs) and human intestinal epithelial lines (Caco-2) cells. Stem Cells Int. 2020, 8835813.
<https://doi.org/10.1155/2020/8835813>
9. , G., Abduldayeva, A., Dosmambetova, K. (2018) Aging biomarkers for evaluating the life style quality of elderly and senile people. Iran. J. Public Health 47(5), 757–758.
10. , T., Pavlova, D., Filchev, R., Dimova, R., Dimov, D., Kavaldzhieva, K., Mrugalska, B. (2023) Technological and human approach in complete restoration of the dental teeth jaws with biocompatible materials. Lecture Notes in Networks and Systems 645, 497–506.
<https://doi.org/10.1007/978-981-99-0769-4_43>
11. , M., Schaller, B., De Almeida Barros Mourão, C. F., Zhang, Y., Sculean, A., Miron, R. J. (2020) Biological characterization of an injectable platelet-rich fibrin mixture consisting of autologous albumin gel and liquid platelet-rich fibrin (Alb-PRF). Platelets 32(1), 74–81.
<https://doi.org/10.1080/09537104.2020.1717455>
12. , K., Kuroda, T., Tamura, N., Mita, H., Kasashima, Y. (2020) Optimal activation methods for maximizing the concentrations of platelet-derived growth factor-BB and transforming growth factor-β1 in equine platelet-rich plasma. J. Vet. Med. Sci. 82(10), 1472–1479.
<https://doi.org/10.1292/jvms.20-0167>
13. , S. B., Hafez, M. H., El-Sayed, Y. S. (2020) Platelet-rich plasma and/or sildenafil topical applications accelerate and better repair wound healing in rats through regulation of proinflammatory cytokines and collagen/TGF-β1 pathway. Environ. Sci. Pollut. Res. Int. 27(32), 40757–40768.
<https://doi.org/10.1007/s11356-020-10042-5>
14. , P., Garcovich, S. (2020) Systematic review – The potential implications of different platelet-rich plasma (PRP) concentrations in regenerative medicine for tissue repair. Int. J. Mol. Sci. 21(16), 5702.
<https://doi.org/10.3390/ijms21165702>
15. , N., Yaneva, G., Ivanov, D. (2021) Characteristics of autologous platelet-rich plasma concentrates obtained by the buffy coat technique using different protocols. Scripta Scientifica Pharmaceutica 8(1), 17–23.
16. , K. E., Mukhamedkerim, K. B., Muradov, M. I., Nabiyev, Y., Turdalieva, B. S. (2023) Restoring function of the flexor tendons of the hand: State of the science in Kazakhstan. Plast. Aesthet. Nurs. (Phila.) 43(4), 219–224.
<https://doi.org/10.1097/PSN.0000000000000530>
17. , J. I., Bae, H. C., Park, H., Lee, M. C., Han, H. S. (2019) Effect of storage conditions and activation on growth factor concentration in platelet-rich plasma. J. Orthop. Res. 38(4), 777–784.
<https://doi.org/10.1002/jor.24520>
18. , M., Harvanová, D., Slovinská, L., Matuška, M., Balog, M., Lacková, A., Špaková, T., Rosocha, J. (2021) Effect of intra-articular injection of platelet-rich plasma on the serum levels of osteoarthritic biomarkers in patients with unilateral knee osteoarthritis. J. Clin. Med. 10(24), 5801.
<https://doi.org/10.3390/jcm10245801>
19. , Y., Song, P., He, J., Liu, B., Liu, S., Zhou, Y., Wang, J. (2022) Comparison between injectable platelet-rich fibrin and platelet-rich plasma in ameliorating UVA-induced photoaging in human dermal fibroblasts via the activation of TGF-β/SMAD signaling pathway. Photochem. Photobiol. 98(6), 1395–1401.
<https://doi.org/10.1111/php.13628>
20. , I., Dakhno, L., Bursova, V. (2025) Effectiveness of topical application with dexamethasone during sagittal split osteotomy of the mandible in minimising clinical symptoms of postoperative neurosensory disorders. BMC Surg. 25(1), 76.
<https://doi.org/10.1186/s12893-025-02803-1>
21. , E., Pulsatelli, L., Cattini, L., Dolzani, P., Assirelli, E., Cenacchi, A., Di Martino, A., Arciola, C. R., Filardo, G. (2023) Pure platelet and leukocyte-platelet-rich plasma for regenerative medicine in orthopedics – Time- and preparation-dependent release of growth factors and effects on synovial fibroblasts: A comparative analysis. Int. J. Mol. Sci. 24(2), 1512.
<https://doi.org/10.3390/ijms24021512>
22. , K., Yuniawati, F., Herawati, D., Purwanti, N., Oktarina, D. A. M. (2019) Effect of freeze-drying process of collagen-activated platelet-rich plasma on transforming growth factor-β1 level. Majalah Kedokteran Gigi Indonesia 5(2), 82–85.
<https://doi.org/10.22146/majkedgiind.40197>
23. , E., Baizakov, A., Kashikova, K., Askerov, R., Argynbayev, Z., Bissaliyev, B. (2023) A new approach to arthroscopic stitching of the knee joint meniscus: A mathematical justification. Med. J. Islam. Repub. Iran 37(1), 108.
24. , Y., Matsushita, T., Nagai, K., Araki, D., Hoshino, Y., Kuroda, R. (2023) Fibrin clot and leukocyte-rich platelet-rich fibrin show similar release kinetics and amount of growth factors: A pilot study. J. Orthop. Surg. Res. 18(1), 97.
<https://doi.org/10.1186/s13018-023-03709-5>
25. , D. V., Bagattini, Â. M., De Almeida, A. M., Mendrone-Júnior, A., Riera, R. (2020) Technical procedures for preparation and administration of platelet-rich plasma and related products: A scoping review. Front. Cell Dev. Biol. 8, 598816.
<https://doi.org/10.3389/fcell.2020.598816>
26. , N., Westbrock, F., Grad, S., Alini, M., Stoddart, M. J., Schmal, H., Kubosch, D., Salzmann, G., Kubosch, E. J. (2021) Evaluation of the influence of platelet-rich plasma (PRP), platelet lysate (PL) and mechanical loading on chondrogenesis in vitro. Sci. Rep. 11(1), 21015.
<https://doi.org/10.1038/s41598-021-99614-0>
27. , Y., Ma, Y., Banchev, A., Duan, W., Xu, P., Zhao, L., Jiang, M., Yu, Z., Zhou, F., Guo, J. J. (2025) Macrophage membrane-encapsulated miRNA nanodelivery system for the treatment of hemophilic arthritis. J. Control. Release 377, 632–647.
<https://doi.org/10.1016/j.jconrel.2024.11.034>
28. , R., Aggarwal, V., Choudhary, A., Basu, D., Nair, N. S., Ganesan, P., Dubashi, B., Govind, R., Kayal, S. (2024) Peripheral blood neutrophil nadir and time to platelet recovery during induction chemotherapy: Predictors of clinical outcomes and markers for optimizing induction treatment intensity in acute lymphoblastic leukemia. Asian Pac. J. Cancer Prev. 25(9), 3229–3237.
<https://doi.org/10.31557/APJCP.2024.25.9.3229>
29. , A. E., Gisbert, S., Palazón, A., Alio, J. L. (2020) Quantification of growth factors and fibronectin in diverse preparations of platelet-rich plasma for the treatment of ocular surface disorders (E-PRP). Transl. Vis. Sci. Technol. 9(6), 22.
<https://doi.org/10.1167/tvst.9.6.22>
30. , H., Gönen, Z. B., Özcan, S., Bahar, D., Lekesizcan, A., Taheri, S., Kütük, N., Alkan, A. (2019) Effects of combination TGF-β1 transfection and platelet-rich plasma (PRP) on three-dimension chondrogenic differentiation of rabbit dental pulp-derived mesenchymal stem cells. Connect. Tissue Res. 62(2), 226–237.
<https://doi.org/10.1080/03008207.2019.1675649>
31. , M., Ruiz, J. M., Pinel, D. M., Sánchez, P., Fiz, N., Guadilla, J., Azofra, J., Beitia, M., Delgado, D. (2025) Increasing the concentration of plasma molecules improves the biological activity of platelet-rich plasma for tissue regeneration. Sci. Rep. 15(1), 11526.
32. , G., Lopreiato, M., Lollobrigida, M., Lamazza, L., Mazzucchi, G., Fortunato, L., Mariano, A., D’Abusco, A. S., Fontana, M., De Biase, A. (2020) Platelet-rich fibrin (PRF) and its related products: Biomolecular characterization of the liquid fibrinogen. J. Clin. Med. 9(4), 1099.
<https://doi.org/10.3390/jcm9041099>
33. , Z. V. (2022) The effect of mechanical skin damage on manifestations of endotoxicosis and immune response under the influence of skeletal trauma complicated by acute blood loss and PRP-therapy effectiveness. Bull. Med. Biol. Res. 4(1), 95–102.
<https://doi.org/10.11603/bmbr.2706-6290.2022.1.12977>
34. , N., Pan, K., Chen, L., Jin, K. (2022) Platelet-derived vesicles enhance the TGF-beta signaling pathway of M1 macrophage. Front. Endocrinol. (Lausanne) 13, 868893.
<https://doi.org/10.3389/fendo.2022.868893>
35. , R., Chellini, F., Idrizaj, E., Tani, A., Garella, R., Pancani, S., Pavan, P., Bambi, F., Zecchi-Orlandini, S., Sassoli, C. (2020) Platelet-rich plasma modulates gap junction functionality and connexin 43 and 26 expression during TGF-β1-induced fibroblast to myofibroblast transition: Clues for counteracting fibrosis. Cells 9(5), 1199.
<https://doi.org/10.3390/cells9051199>
36. , R. V., Haldankar, P., Joshi, V. R., Raj, R., Maradi, R. (2022) Variability in platelet-rich plasma preparations used in regenerative medicine: A comparative analysis. Stem Cells Int. 2022, 3852898.
37. , J., Li, X. J., Ma, Y., Mai, Z., Yang, Y., Luo, M., Xu, W., Chen, K., Chen, X., Tang, J., Cheng, B., Cui, X. (2023) Correlation of bioactive components of platelet-rich plasma derived from human female adult peripheral blood and umbilical cord blood with age. Sci. Rep. 13(1), 18428.
<https://doi.org/10.1038/s41598-023-45747-3>
38. , T., Kokovin, M., Drozd, P., Prylutska, S. (2023) Identification of mycoplasma contamination in cell culture. Biol. Syst. Theory Innov. 14(2), 76–83.
39. , W., Yu, T., Chang, G., Chang, H., Lin, L., Lin, M., Pang, J. S. (2021) Use of platelet-rich plasma plus suramin, an antifibrotic agent, to improve muscle healing after injuries. Am. J. Sports Med. 49(11), 3102–3112.
<https://doi.org/10.1177/03635465211030295>
40. , D. C. D., Yambay, C. F. P., Bermúdez, V., Zabaleta, A. K. C., Gotera, J. (2025) D-dimer, platelets and C-reactive protein in patients with COVID-19: Correlations in deceased and surviving patients. Gac. Med. Caracas 133(1), 17–27.
<https://doi.org/10.47307/GMC.2025.133.1.2>
41. World Medical Association (2025) WMA Declaration of Helsinki – Ethical principles for medical research involving human participants. WMA Declaration of Helsinki. Available at: https://www.wma.net/policies-post/wma-declaration-of-helsinki/
42. , Y., Liu, X., Chen, Y., He, M., Xie, J., Xiao, G. (2024) Effects of platelet-rich plasma combined with physical therapy on IL-1β, TGF-β1, and MMP-3 in patients with knee osteoarthritis. Mol. Biotechnol. 67, 1991–2001.
<https://doi.org/10.1007/s12033-024-01177-8>
43. , R. F., Rezaei-Tavirani, M., Oskouie, A. A., Amiri-Dashatan, N. (2025) Biomarker panels associated with diagnosis and overall survival in hepatocellular carcinoma revealed from protein-protein and mRNA-miRNA interaction networks. Asian Pac. J. Cancer Prev. 26(1), 249–262.
<https://doi.org/10.31557/APJCP.2025.26.1.249>
